Device

Part:BBa_K2018000:Design

Designed by: Jakob Rnning & Joel   Group: iGEM16_SDU-Denmark   (2016-05-20)


phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 979
    Illegal BglII site found at 1804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 285
    Illegal NgoMIV site found at 356
    Illegal NgoMIV site found at 956
    Illegal NgoMIV site found at 1268
    Illegal NgoMIV site found at 1547
    Illegal NgoMIV site found at 2199
    Illegal NgoMIV site found at 2221
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4065


Design Notes

This part is created as part of an experiment to improve the phaCAB biobrick and understand how additional promoter and RBS sites improve yield.


Source

Bla bla bla

References